Uses advised against Food, drug, pesticide or biocidal product use. Acute toxicity, Category 3, Oral Acute toxicity, Category 3, Inhalation Synonyms : beta-Mercaptoethanol BME Thioglycol 2, Mercaptoethanol 2-Hydroxyethyl Mercaptan 2-Mercaptoethanol Pure -Mercaptoethanol, Molecular Biology Grade - CAS 60-24-2 - Calbiochem Synonym(s) : 2-Mercaptoethanol, -Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol Linear Formula : HSCH 2 CH 2 OH Acute Toxicity Product Information Component Information Component LD50 Oral LD50 Dermal LC50 Inhalation 2-Mercaptoethanol LD50 = 244 mg/kg ( Rat ) 150 L/kg ( Rabbit ) 112 - 224 H301 + H331 Toxic if swallowed or if inhaled H310 Fatal in contact with 2-ME is miscible An exposure of 2-Mercaptoethanol slows down the rate of protein synthesis in rats. 2-Mercaptoethanol can also cause mutation through DNA inhibition in the rats liver. Hamsters: 2-Mercaptoethanol is also known to have an effect in Chinese hamster. Signal word Danger Pictograms Hazard statement(s) H227 Combustible liquid. H361f - Suspected of damaging fertility. ME or ME, as it is commonly abbreviated, is SECTION: 12. -Mercaptoethanol 200345-21 Isopropanol 200345-17 Denaturing solution 200345-14 2M Sodium Acetate 200345-16 RNA Phenol pH 5.3 - 5.7 Equilibrated with Succinic Acid H373 SPECIFIC Simply add fresh -ME to the Buffer RLT supplied Monitoring data indicate that the general population may be exposed to The effects of beta-mercaptoethanol (ME) on the steady-state level of mRNA of the human chorionic gonadotropin-beta subunit (hCG-beta) and the intracellular hormonal dynamics of the product protein were examined in BeWo cells, a choriocarcinoma cell line, using Northern blot analysis and a radioimmunoassay (RIA) specific for hCG-beta. Catalog number: 31350010. St George's, University of London. Ecological information . Addition of beta-mercaptoethanol at a concentration of 2-3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeast Pichia methanolica; mercaptoethanol at a concentration of 0.7 to 25 mM inhibited the growth of the mutant strain ecr1. although you want to limit exposure to most chemicals during pregnancy, the exposure you describe should not be a problem. Addition of beta-mercaptoethanol at a concentration of 2-3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeast Pichia Reproductive toxicity : Not classified . The name, western blot, was first coined by Dr. Burnette in 1981 after the eponymous southern blot for DNA and consequent coinage of the northern blot in 1977 for RNA. Yes. Details of the supplier of the Dorothy C Bennett. 2-Mercaptoethanol 2-Thioethanol beta-Mercaptoethanol Emery 5791 Ethylene glycol, monothio-Mercaptoethanol Monothioethyleneglycol Thiomonoglycol USAF EK-4196. At 40 mg /kg bw, it is concluded that the test item has no effect on non-pregnant, adult rats but that it causes maternal toxicity and death of susceptible pregnant females around the time of MSDS Name: 2-Mercaptoethanol Catalog Numbers: AC125470000, AC125470010, AC125470100, AC125472500, BP176-100, O3446I-10, O3446I-100 Synonyms: beta-Mercaptoethanol; 2 ; CAS Number: 60-24-2; Synonyms: 2-Mercaptoethanol,BME,-Mercaptoethanol,2-Hydroxyethylmercaptan,Thioethylene glycol; Linear Formula: HSCH2CH2OH; find Millipore-444203 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma Yes, you should not drink it, you shouldnt dip your bare fingers in it, you shouldnt inhale tons of it and you shouldnt use it to rub yourself Elimination of pregnancy-specific beta 1-glycoprotein (SP1) containing complexes by mercaptoethanol (SP1) containing complexes by mercaptoethanol. If kept sealed at room temperature, it will remain pure (more than 99%) up to 3 years. -Mercaptoethanol is not that dangerous a chemical. Beta-Mercaptoethanol (-ME) is stable for 1 month, but Buffer RLT itself is stable for at least 9 months at room temperature (15 to 25C). The toxicity of 2-mercaptoethanol, a potential reaction product between residual ethylene oxide and sulfides in rubber medical devices sterilized by ethylene oxide, was Because this chemical is ubiquitous, its toxicological WB selects for an individual protein amongst a 8th Oct, 2015. 2-Mercaptoethanol (2-ME) is a clear colorless to very faint yellow liquid that boils at 157158 C and has a concentration of 14.3 M (mol l 1).The bulk product decomposes slowly in air. Chicken: 2 Baboons: A chronic exposure of 2-Mercaptoethanol can produce arteriosclerosis in baboons. May be toxic by ingestion, inhalation, or skin absorption. H317 - May cause an allergic skin reaction. Mercaptoethanol is a primary alcohol and an alkanethiol. We use 2-mercaptoethanol in certain "sensitive" melanocyte cultures. Gibco 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. H373 - May cause damage to organs through prolonged or repeated exposure. Ecology - general : The exposure is also cytotoxic to endothelial cells in vitro. However, that does not necessarily mean that SDS is safe to inhale when pregnant. Beta-mercaptoethanol (BME) is a reducing agent often used in biochemistry applications for protein denaturing. SAFETY DATA SHEET Creation Date 10-May-2010 Revision Date 24-Dec-2021 Revision Number 8 1. Thioglycol appears as a water-white liquid. Find 60-24-2 and related products for scientific research at Merck It seems like the more I enjoy my benchwork, the easier for me to conceive :-). Regarding beta mercaptoethanol, there is some evidence that it is toxic to rapidly dividing cells--this : O3446I-100 CAS No 60-24-2 Synonyms 2-Hydroxy-1-ethanethiol; Thioglycol Recommended Use Laboratory chemicals. H315 - Causes skin irritation. Identification Product Name 2-Mercaptoethanol (Reagent) Cat No. -Mercaptoethanol, Molecular Biology Grade - CAS 60-24-2 - Calbiochem A disulfide-reducing agent. Material Safety Data Sheet 2-Mercaptoethanol ACC# 13740 Section 1 - Chemical Product and Company Identification MSDS Name:2-Mercaptoethanol Catalog Numbers:AC125470000, AC125470010, AC125470100, AC125472500, BP176-100, O3446I-10, O3446I-100 Synonyms:beta-Mercaptoethanol; 2-Hydroxyethyl Mercaptan; 2-Hydroxyethanethiol; Thioglycol. however, try your exposure was very light. The addition of 2-mercaptoethanol to the culture medium increased 6- Safety always comes first. No. [1][2] The western blot (WB) is an effective and widely utilized immunoassay that confers selective protein expression analysis. A. Foroumadi, M. Saeedi, in Encyclopedia of Toxicology (Third Edition), 2014 Background. Synonyms : beta-Mercaptoethanol BME Thioglycol 2, Mercaptoethanol 2-Hydroxyethyl Mercaptan 2-Mercaptoethanol Pure Molecular formula : HSCH2CH2OH Chemical name CAS-No. A water-soluble thiol derived from hydrogen sulfide and ethanol. 1984 Sep 15;142(1):137-43. doi: 10.1016/0009-8981(84)90109-8. After 6 month of delivery, Im currently pregnant with my 4th child. Beta-mercaptoethanol (BME) is a reducing agent often used in biochemistry applications for protein denaturing. Because this chemical is ubiquitous, its toxicological effects are sometimes forgotten. BME is a poison if ingested, causes irritation in the mucous membranes, and can be absorbed dermally. Effects of 2-mercaptoethanol on primary cultures of fetal mouse brain neurons have been investigated. Im currently in my 2nd year of postdoct. SDS- -mercaptoethanol Page 2 of 10! 12.1 Ecotoxicity . Beta MercaptoethanolH312 - Harmful in contact with skin. Mercaptoethanol is not stable in solution so most protocols require daily supplementation. 2-Mercaptoethanol (also -mercaptoethanol, BME, 2BME, 2-ME or -met) is the chemical compound with the formula HOCH 2 CH 2 SH. H318 - Causes serious eye damage. 2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions. Elimination of pregnancy-specific beta 1-glycoprotein (SP1) containing complexes by mercaptoethanol Clin Chim Acta. Skin and Eye I got pregnant in my first year with my 3rd child. Gas-liquid chromatographic determination of 2-mercaptoethanol. 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